T-DNA locus structure in a large population of soybean plants transformed using the Agrobacterium-mediated cotyledonary-node method
نویسندگان
چکیده
منابع مشابه
bialaphos-resistant transgenic soybeans produced by the agrobacterium-mediated cotyledonary-node method
a stable agrobacterium-mediated transformation system was established using bialaphos as the selective agent in soybeans [glycine max (l.) merr.]. the cotyledonary node explants of the soybean cultivar ‘ny-1001’ were inoculated with the agrobacterium tumefaciens strain eha105, harboring the vector pcambia3301 containing the gus gene as the reporter gene and the bar gene conferring bialaphos res...
متن کاملBialaphos-resistant Transgenic Soybeans Produced by the Agrobacterium-mediated Cotyledonary-node Method
A stable Agrobacterium-mediated transformation system was established using bialaphos as the selective agent in soybeans [Glycine max (L.) Merr.]. The cotyledonary node explants of the soybean cultivar ‘NY-1001’ were inoculated with the Agrobacterium tumefaciens strain EHA105, harboring the vector pCAMBIA3301 containing the gus gene as the reporter gene and the bar gene conferring bialaphos res...
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A reproducible and highly efficient protocol for genetic transformation mediated by Agrobacterium has been established for greengram (Vigna radiata L. Wilczek). Double cotyledonary node (DCN) explants were inoculated with Agrobacterium tumefaciens strain LBA 4404 harboring a binary vector pCAMBIA 2301 containing neomycin phosphotransferase (npt II) gene as selectable marker, β-glucuronidase (GU...
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در سال های اخیر، تقاضای استفاده از تئوری خطی ویسکوالاستیسیته بیشتر شده است. با افزایش استفاده از کامپوزیت های پیشرفته در صنایع هوایی و همچنین استفاده روزافزون از مواد پلیمری، اهمیت روش های دقیق طراحی و تحلیل چنین ساختارهایی بیشتر شده است. این مواد جدید از خودشان رفتارهای مکانیکی ارائه می دهند که با تئوری های الاستیسیته و ویسکوزیته، نمی توان آن ها را توصیف کرد. این مواد، خواص ویسکوالاستیک دارند....
Agrobacterium-mediated transformation of cotyledonary leaf of lettuce (Lactuca sativa L.) by the GCHI gene
Lactuca sativa is widely used as a leafy vegetable belonging to the Asteraceae family. Cotyledonary leaves of in vitro lettuce seedlings were transformed by plasmids containing the GCHI gene. Also the effects of number of days of pre-cultivation and co-cultivation of explants were examined on the transformation frequency. After co-cultivation, the explants were transferred onto the regeneration...
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ژورنال
عنوان ژورنال: Plant Biotechnology Journal
سال: 2004
ISSN: 1467-7644,1467-7652
DOI: 10.1111/j.1467-7652.2004.00070.x